D-hydantoinase相关论文
D-p-hydroxyphenylglycine production from DL-p-hydroxyphenylhydantoin using the whole cells in aqueou
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Hydantoinase and N-carbamoylase play important roles in the production of optically pure amino acids from racemic 5-mono......
Cloning and nucleotide sequence of D-hydantoinase gene of marine polyphosphate-accumulating bacteriu
为探究吕家坨井田地质构造格局,根据钻孔勘探资料,采用分形理论和趋势面分析方法,研究了井田7......
Gene Sequence, Soluble Expression and Homologous Comparison of a D-Hydantoinase from Pseudomonas put
为探究吕家坨井田地质构造格局,根据钻孔勘探资料,采用分形理论和趋势面分析方法,研究了井田7......
为实现利用生物酶转化法进行D-对羟基苯甘氨酸的工业化生产,构建了3株海因酶基因工程菌.利用PCR技术从恶臭假单胞菌(Pseudomonas p......
以中华根瘤菌(Sinorhizobium morelense)SS—ori总DNA为模板,用PCR法扩增D-海因酶基因,分别克隆到5种不同的载体,并转入5种不同的Esch......
报道了一株假单胞杆菌(Pseudomonas putida YZ-Ⅱ6)的形态特征及产海因酶的发酵条件.结果表明该菌为杆状,菌落圆形光滑,革兰氏染色......
D-对羟基苯甘氨酸(D-p-HPG)是半合成青霉素和头孢霉素的重要前体.综述了微生物酶法生产D-对羟基苯甘氨酸的方法种类及其优缺点并对......
介绍了酶法生产D-对羟基苯甘氨酸的研究现状.综述了酶法生产D-对羟基苯甘氨酸的方法种类及其优缺点并对酶的来源即菌种的筛选方法......
目的采用多种筛选方法以获得D-海因酶及N-氨甲酰水解酶的阳性菌株.方法通过以底物(对羟基苯海因)为唯一氮源的培养基从土样和水样......
为实现利用生物酶转化法进行D-对羟基苯甘氨酸的工业化生产,构建了3株海因酶基因工程菌。利用PCR技术从恶臭假单胞菌(Pseudomonas put......
利用产D-海因酶(HDT)的重组pE-hdt/E.coli菌株,在LB培养基中添加40μmol/L的Co^2+,37℃培养10h,表达产物经Q-Sepharose阴离子交换剂和......
通过PCR分别扩增得到N-carbamoylase基因和上游带有RBS区的D—hydantoinase基因,并将其依次克隆入pBAD/His A质粒中,得到呈多顺反子结......
报道D-海因酶分子C-末端Arg残基的缺失,导致酶的解聚与稳定性的显著改变。用基因克隆、表达与纯化的方法,制备了重组D-海因酶(P479)及......
利用自行筛选的Pseudomonas sp.js-01菌发酵产酶.得到兼有海因酶.N-氨甲酰水解酶活性的菌体,通过酶动力学参数分析以及转化试验表明.N-......
Purification and Biochemical Characterization of D-hydantoinase and D-N-carbamoylase from Burkholder
Hydantoinase and N-carbamoylase play important roles in the production of optically pure amino acids from racemic 5-mono......
Gene Sequence, Soluble Expression and Homologous Comparison of a D-Hydantoinase from Pseudomonas put
A 1440bp open-reading frame encoding D-hydantoinase from Pseudomonas putida YZ-26 was cloned and sequenced(GenBank AY387......